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htert immortalized foreskin fibroblast cell line bj 5ta  (ATCC)


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    ATCC htert immortalized foreskin fibroblast cell line bj 5ta
    TgApMFS1 is required for the survival of parasites. ( a ) Plaque assays were performed by infecting <t>BJ-5ta</t> cells with TgApMFS1 loxp parasites for 8 days in the presence or absence of rapamycin. WT, wild type; ( b ) Replication of TgApMFS1 loxp parasites. The parasites were treated with or without rapamycin for 72 h, then inoculated into BJ-5ta cells in 12-well plates and allowed to grow with or without rapamycin for 24 h. The average number of parasites per vacuole was determined. The data were analyzed by a two-way ANOVA; **** p < 0.0001.
    Htert Immortalized Foreskin Fibroblast Cell Line Bj 5ta, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 489 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/htert immortalized foreskin fibroblast cell line bj 5ta/product/ATCC
    Average 98 stars, based on 489 article reviews
    htert immortalized foreskin fibroblast cell line bj 5ta - by Bioz Stars, 2026-02
    98/100 stars

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    1) Product Images from "A Major Facilitator Superfamily Transporter Is Critical for the Metabolism and Biogenesis of the Apicoplast"

    Article Title: A Major Facilitator Superfamily Transporter Is Critical for the Metabolism and Biogenesis of the Apicoplast

    Journal: Pathogens

    doi: 10.3390/pathogens14080763

    TgApMFS1 is required for the survival of parasites. ( a ) Plaque assays were performed by infecting BJ-5ta cells with TgApMFS1 loxp parasites for 8 days in the presence or absence of rapamycin. WT, wild type; ( b ) Replication of TgApMFS1 loxp parasites. The parasites were treated with or without rapamycin for 72 h, then inoculated into BJ-5ta cells in 12-well plates and allowed to grow with or without rapamycin for 24 h. The average number of parasites per vacuole was determined. The data were analyzed by a two-way ANOVA; **** p < 0.0001.
    Figure Legend Snippet: TgApMFS1 is required for the survival of parasites. ( a ) Plaque assays were performed by infecting BJ-5ta cells with TgApMFS1 loxp parasites for 8 days in the presence or absence of rapamycin. WT, wild type; ( b ) Replication of TgApMFS1 loxp parasites. The parasites were treated with or without rapamycin for 72 h, then inoculated into BJ-5ta cells in 12-well plates and allowed to grow with or without rapamycin for 24 h. The average number of parasites per vacuole was determined. The data were analyzed by a two-way ANOVA; **** p < 0.0001.

    Techniques Used:

    Depletion of TgApMFS1 disturbs fatty acid synthesis in the parasites. ( a ) GC-MS analysis of fatty acids in parasites lacking TgApMFS1. BJ-5ta cells were cultured with 13C-glucose for 6 h, inoculated with parasites, and then cultured with rapamycin for an additional 48 h. The egressed parasites were collected, purified using a 5 µm filter, and analyzed by GC-MS. The wild type parasites cultured with rapamycin were used as control in this study; **** p < 0.0001, * p = 0.0136, * p = 0.035; ( b ) Mass isotopologue distributions (MID) of fatty acid (FA) (C14:0) from 13 C-glucose in the parasites lacking TgApMFS1. The x -axis indicates the number of 13 C atoms in each Fatty Acid Methyl Ester (FAME), where ‘m0′ indicates the monoisotopic mass containing no 13 C atoms, while ‘mX’ represents that mass with ‘X’ 13C atoms incorporated. **** p < 0.0001, **** p < 0.0001, * p = 0.0143, **** p < 0.0001, **** p < 0.0001, **** p < 0.0001, **** p < 0.0001, **** p < 0.0001, **** p < 0.0001, **** p < 0.0001, * p = 0.013.
    Figure Legend Snippet: Depletion of TgApMFS1 disturbs fatty acid synthesis in the parasites. ( a ) GC-MS analysis of fatty acids in parasites lacking TgApMFS1. BJ-5ta cells were cultured with 13C-glucose for 6 h, inoculated with parasites, and then cultured with rapamycin for an additional 48 h. The egressed parasites were collected, purified using a 5 µm filter, and analyzed by GC-MS. The wild type parasites cultured with rapamycin were used as control in this study; **** p < 0.0001, * p = 0.0136, * p = 0.035; ( b ) Mass isotopologue distributions (MID) of fatty acid (FA) (C14:0) from 13 C-glucose in the parasites lacking TgApMFS1. The x -axis indicates the number of 13 C atoms in each Fatty Acid Methyl Ester (FAME), where ‘m0′ indicates the monoisotopic mass containing no 13 C atoms, while ‘mX’ represents that mass with ‘X’ 13C atoms incorporated. **** p < 0.0001, **** p < 0.0001, * p = 0.0143, **** p < 0.0001, **** p < 0.0001, **** p < 0.0001, **** p < 0.0001, **** p < 0.0001, **** p < 0.0001, **** p < 0.0001, * p = 0.013.

    Techniques Used: Gas Chromatography-Mass Spectrometry, Cell Culture, Purification, Control



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    Image Search Results


    TgApMFS1 is required for the survival of parasites. ( a ) Plaque assays were performed by infecting BJ-5ta cells with TgApMFS1 loxp parasites for 8 days in the presence or absence of rapamycin. WT, wild type; ( b ) Replication of TgApMFS1 loxp parasites. The parasites were treated with or without rapamycin for 72 h, then inoculated into BJ-5ta cells in 12-well plates and allowed to grow with or without rapamycin for 24 h. The average number of parasites per vacuole was determined. The data were analyzed by a two-way ANOVA; **** p < 0.0001.

    Journal: Pathogens

    Article Title: A Major Facilitator Superfamily Transporter Is Critical for the Metabolism and Biogenesis of the Apicoplast

    doi: 10.3390/pathogens14080763

    Figure Lengend Snippet: TgApMFS1 is required for the survival of parasites. ( a ) Plaque assays were performed by infecting BJ-5ta cells with TgApMFS1 loxp parasites for 8 days in the presence or absence of rapamycin. WT, wild type; ( b ) Replication of TgApMFS1 loxp parasites. The parasites were treated with or without rapamycin for 72 h, then inoculated into BJ-5ta cells in 12-well plates and allowed to grow with or without rapamycin for 24 h. The average number of parasites per vacuole was determined. The data were analyzed by a two-way ANOVA; **** p < 0.0001.

    Article Snippet: The parasites were cultured on the hTERT-immortalized foreskin fibroblast cell line BJ-5ta (ATCC ® CRL-4001) in Dulbecco’s Modified Eagle Medium (DMEM) + 10% Fetal Bovine Serum (FBS).

    Techniques:

    Depletion of TgApMFS1 disturbs fatty acid synthesis in the parasites. ( a ) GC-MS analysis of fatty acids in parasites lacking TgApMFS1. BJ-5ta cells were cultured with 13C-glucose for 6 h, inoculated with parasites, and then cultured with rapamycin for an additional 48 h. The egressed parasites were collected, purified using a 5 µm filter, and analyzed by GC-MS. The wild type parasites cultured with rapamycin were used as control in this study; **** p < 0.0001, * p = 0.0136, * p = 0.035; ( b ) Mass isotopologue distributions (MID) of fatty acid (FA) (C14:0) from 13 C-glucose in the parasites lacking TgApMFS1. The x -axis indicates the number of 13 C atoms in each Fatty Acid Methyl Ester (FAME), where ‘m0′ indicates the monoisotopic mass containing no 13 C atoms, while ‘mX’ represents that mass with ‘X’ 13C atoms incorporated. **** p < 0.0001, **** p < 0.0001, * p = 0.0143, **** p < 0.0001, **** p < 0.0001, **** p < 0.0001, **** p < 0.0001, **** p < 0.0001, **** p < 0.0001, **** p < 0.0001, * p = 0.013.

    Journal: Pathogens

    Article Title: A Major Facilitator Superfamily Transporter Is Critical for the Metabolism and Biogenesis of the Apicoplast

    doi: 10.3390/pathogens14080763

    Figure Lengend Snippet: Depletion of TgApMFS1 disturbs fatty acid synthesis in the parasites. ( a ) GC-MS analysis of fatty acids in parasites lacking TgApMFS1. BJ-5ta cells were cultured with 13C-glucose for 6 h, inoculated with parasites, and then cultured with rapamycin for an additional 48 h. The egressed parasites were collected, purified using a 5 µm filter, and analyzed by GC-MS. The wild type parasites cultured with rapamycin were used as control in this study; **** p < 0.0001, * p = 0.0136, * p = 0.035; ( b ) Mass isotopologue distributions (MID) of fatty acid (FA) (C14:0) from 13 C-glucose in the parasites lacking TgApMFS1. The x -axis indicates the number of 13 C atoms in each Fatty Acid Methyl Ester (FAME), where ‘m0′ indicates the monoisotopic mass containing no 13 C atoms, while ‘mX’ represents that mass with ‘X’ 13C atoms incorporated. **** p < 0.0001, **** p < 0.0001, * p = 0.0143, **** p < 0.0001, **** p < 0.0001, **** p < 0.0001, **** p < 0.0001, **** p < 0.0001, **** p < 0.0001, **** p < 0.0001, * p = 0.013.

    Article Snippet: The parasites were cultured on the hTERT-immortalized foreskin fibroblast cell line BJ-5ta (ATCC ® CRL-4001) in Dulbecco’s Modified Eagle Medium (DMEM) + 10% Fetal Bovine Serum (FBS).

    Techniques: Gas Chromatography-Mass Spectrometry, Cell Culture, Purification, Control